PITX2 Antibody

Pilx2 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. It plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this protein are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:1000

UniProt

Q99697

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 122-151 from the human protein was used as the immunogen for this PITX2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PITX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PITX2 antibody is available for research use only.

Storage Conditions

Aliquot the PITX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PITX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Chicken, Zebrafish, Xenopus

Image Legend

Fluorescent image of HeLa cell stained with PITX2 antibody. Alexa Fluor 488 conjugated secondary (green) was used. Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin. PITX2 immunoreactivity is localized to the nucleus.