CD8 Antibody

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P01732

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 150-180 from the human protein was used as the immunogen for this CD8 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CD8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD8 antibody is available for research use only.

Storage Conditions

Aliquot the CD8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Flow cytometric analysis of NCI-H292 cells using CD8 antibody (bottom histogram) compared to a negative control (top histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.