GATA4 Antibody

GATA4 is a transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP) -mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

P43694

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 298-328 from the human protein was used as the immunogen for this GATA4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GATA4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GATA4 antibody is available for research use only.

Storage Conditions

Aliquot the GATA4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the GATA4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Xenopus

Image Legend

GATA4 antibody flow cytometric analysis of HepG2 cells (green) compared to a negative control (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.