VASP Antibody / Vasodilator stimulated phosphoprotein
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
P50552
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 267-296 from the human protein was used as the immunogen for this VASP antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the VASP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This VASP antibody is available for research use only.
Storage Conditions
Aliquot the VASP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the VASP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
VASP antibody western blot analysis with 1) HUVEC and 2) THP-1 lysate. Expected molecular weight: ~40/46-50 kDa (unmodified/phosphorylated) .
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