TLR9 Antibody

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

Q9NR96

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 842-870 from the human protein was used as the immunogen for this TLR9 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TLR9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TLR9 antibody is available for research use only.

Storage Conditions

Aliquot the TLR9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the TLR9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

TLR9 antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control (left histogram) . FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.