ABHD4 Antibody
Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE) . Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
Q8TB40
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 98-127 from the human protein was used as the immunogen for this ABHD4 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ABHD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ABHD4 antibody is available for research use only.
Storage Conditions
Aliquot the ABHD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the ABHD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Location
Cytoplasmic
Image Legend
ABHD4 antibody western blot analysis in mouse kidney tissue lysate. Predicted molecular weight ~39 kDa.
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