MMP3 Antibody

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P08254

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 30-59 from the human protein was used as the immunogen for this MMP3 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the MMP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MMP3 antibody is available for research use only.

Storage Conditions

Aliquot the MMP3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the MMP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from 1) HepG2, 2) NCI-H460,3) Raji, and 4) Ramos cell line using MMP3 antibody at 1:1000. Predicted molecular weight ~54kDa.