BNIP3 Antibody
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:50-1:100, IHC (Paraffin) : 1:50-1:100
UniProt
Q12983
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 152-187 from the human protein (BH3 domain) was used as the immunogen for this BNIP3 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the BNIP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This BNIP3 antibody is available for research use only.
Storage Conditions
Aliquot the BNIP3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the BNIP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Fluorescent confocal image of HepG2 cells stained with BNIP3 antibody at 1:50. BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
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