Puma Antibody
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
Q9BXH1
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 119-154 (BH3 domain) from the alpha isoform was used as the immunogen for this Puma antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Puma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Puma antibody is available for research use only.
Storage Conditions
Aliquot the Puma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Puma antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat
Image Legend
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