SUMO2 Antibody

SUMO2 is a ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. SUMO2 does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. This protein plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS isoforms 1-4.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

Q7ZTK7

Host

Rabbit

Reactivity

Xenopus

Immunogen

A portion of amino acids 1-30 from the xenla protein was used as the immunogen for this SUMO2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SUMO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SUMO2 antibody is available for research use only.

Storage Conditions

Aliquot the SUMO2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the SUMO2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of SUMO2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the SUMO2 gene.