MyoD Antibody
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50
UniProt
P15172
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 211-240 from the human protein was used as the immunogen for this MyoD antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the MyoD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MyoD antibody is available for research use only.
Storage Conditions
Aliquot the MyoD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the MyoD antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
MyoD antibody western blot analysis in (1) HeLa cell line and (2) human placenta tissue lysate. Expected molecular weight: 43-45 kDa
Frequently Asked Questions
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