SUMO4 Antibody
Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:50-1:100, Western blot: 1:1000
UniProt
Q6EEV6
Host
Rabbit
Reactivity
Human
Immunogen
This SUMO4 antibody was produced from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human SUMO4.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SUMO4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SUMO4 antibody is available for research use only.
Storage Conditions
Aliquot the SUMO4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SUMO4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of SUMO4 antibody and HepG2 lysate. Observed molecular weight: 11-15 kDa.
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