Anti-MDM2 Antibody

MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

Q00987

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 393-424 from the human protein was used as the immunogen for this anti-MDM2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the anti-MDM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This anti-MDM2 antibody is available for research use only.

Storage Conditions

Aliquot the anti-MDM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the anti-MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of anti-MDM2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (2) .