UBF Antibody (UBF1)
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP and multiple TBP-associated factors or 'TAFs') . Two UBF polypeptides, of 94 and 97 kD, exist in the human. UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P17480
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 631-660 from the human protein was used as the immunogen for this UBF1 / UBF antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the UBF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This UBF antibody is available for research use only.
Storage Conditions
Aliquot the UBF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the UBF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
UBF1 / UBF antibody western blot analysis in K562 lysate.
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