NEDD8 Antibody
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
Q15843
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-32 from the human protein was used as the immunogen for this NEDD8 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the NEDD8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NEDD8 antibody is available for research use only.
Storage Conditions
Aliquot the NEDD8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the NEDD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine, Rabbit
Location
Nuclear, cytoplasmic
Image Legend
Western blot analysis of NEDD8 antibody and HeLa cell lysate. Predicted molecular weight ~9 kDa.
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