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FOXA2 Antibody

FOXA2 is a transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

Q9Y261

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A portion of amino acids 380-407 from the human protein was used as the immunogen for this FOXA2 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC, IF, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the FOXA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FOXA2 antibody is available for research use only.

Storage Conditions

Aliquot the FOXA2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the FOXA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Confocal immunofluorescent analysis of FOXA2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) . DAPI was used as a nuclear counterstain (blue) .

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