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AKT Antibody

In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. [RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P31749

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 428-457 from human AKT1 was used as the immunogen for this AKT antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, FACS, WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the AKT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This AKT antibody is available for research use only.

Storage Conditions

Aliquot the AKT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Rat

Image Legend

AKT antibody western blot analysis in MCF-7 lysate

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