XRCC5 Antibody / Ku80

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V (D) J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V (D) J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P13010

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 424-450 from the human protein was used as the immunogen for this XRCC5 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the XRCC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This XRCC5 antibody is available for research use only.

Storage Conditions

Aliquot the XRCC5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the XRCC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Confocal immunofluorescent analysis of XRCC5 antibody with HeLa cells followed by Alexa Fluor 488-conjugated secondary (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) . DAPI was used to stain the nuclei (blue) .