KLF4 Antibody
KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
O43474
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 394-421 from the human protein was used as the immunogen for this KLF4 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, FACS, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This KLF4 antibody is available for research use only.
Storage Conditions
Aliquot the KLF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
KLF4 antibody western blot analysis in (1) A431 and (2) MCF-7 lysate. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form) .
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