GCLC Antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P48506

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this GCLC antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GCLC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GCLC antibody is available for research use only.

Storage Conditions

Aliquot the GCLC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the GCLC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Confocal immunofluorescent analysis of GCLC antibody with U-251MG cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .