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ENos Antibody / NOS3

ENos/NOS3 produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P29474

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1156-1183 from the human protein was used as the immunogen for this eNos antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the eNos antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This eNos antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the eNos antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the eNos antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Bovine, Pig

Image Legend

ENos antibody western blot analysis in K562 lysate

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