Nucleolin Antibody
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50
UniProt
P19338
Host
Rabbit
Reactivity
Human
Immunogen
The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
The Nucleolin antibody should be aliquoted and and stored frozen at -20oC. Avoid repeat freeze-thaws.
Limitations
This Nucleolin antibody is available for research use only.
Storage Conditions
The Nucleolin antibody should be aliquoted and and stored frozen at -20°C. Avoid repeat freeze-thaws.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Prediction Reactivity
Primate
Image Legend
Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
Frequently Asked Questions
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