Nucleolin Antibody

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50

UniProt

P19338

Host

Rabbit

Reactivity

Human

Immunogen

The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

The Nucleolin antibody should be aliquoted and and stored frozen at -20oC. Avoid repeat freeze-thaws.

Limitations

This Nucleolin antibody is available for research use only.

Storage Conditions

The Nucleolin antibody should be aliquoted and and stored frozen at -20°C. Avoid repeat freeze-thaws.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance.