ADA Antibody
Adenosine deaminase (ADA) catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunohistochemistry (FFPE) : 1:100-1:500
UniProt
P00813
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 287-314 from the human protein was used as the immunogen for this ADA antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ADA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ADA antibody is available for research use only.
Storage Conditions
Aliquot the ADA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the ADA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasm, cell membrane
Image Legend
IHC testing of FFPE human duodenum tissue with ADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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