Osteopontin Antibody

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunohistochemistry (FFPE) : 1:50-1:100, Immunofluorescence: 1:10-1:50

UniProt

P10451

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 14-40 from the human protein was used as the immunogen for this Osteopontin antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Osteopontin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Osteopontin antibody is available for research use only.

Storage Conditions

Aliquot the Osteopontin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Osteopontin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) HeLa, 2) HL60, 3) Jurkat and 4) MOLT4 cell lysate with Osteopontin antibody. Predicted molecular weight: 35-65 kDa depending on degree of glycosylation.