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Oct3/4 Antibody

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t (6;22) (p21; q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

Q01860

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 265-297 from the human protein was used as the immunogen for this Oct3/4 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the Oct3/4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Oct3/4 antibody is available for research use only.

Storage Conditions

Aliquot the Oct3/4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Oct3/4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Oct3/4 antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~38/30kDa (isoform A/B) .

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