HDAC1 Antibody

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50

UniProt

Q13547

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this HDAC1 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HDAC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HDAC1 antibody is available for research use only.

Storage Conditions

Aliquot the HDAC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the HDAC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

HDAC1 antibody western blot analysis of (1) HeLa, (2) K562, (3) mouse NIH3T3 cell line and (4) mouse testis, (5) rat testis and (6) rat spleen tissue lysate. Predicted molecular weight 55~60 kDa