CD11b Antibody / MAC-1 / ITGAM
CD11b is the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3') . The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Western blot: 1:1000
UniProt
P11215
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 253-282 from the human protein was used as the immunogen for this CD11b antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, FACS, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CD11b antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD11b antibody is available for research use only.
Storage Conditions
Aliquot the CD11b antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CD11b antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cell membrane, cytoplasm
Image Legend
Western blot testing of human spleen lysate with CD11b antibody. Expected molecular weight: 128-170 kDa depending on glycosylation level.
Documents
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
