Anti-Histone H3 Antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The protein targeted by this antibody is a replication-independent member of the histone H3 family.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P84243
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this anti-Histone H3 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the anti-Histone H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-Histone H3 antibody is available for research use only.
Storage Conditions
Aliquot the anti-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the anti-Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus, Drosophila, C. elegans
Image Legend
Western blot analysis of anti-Histone H3 in CEM lysate
Frequently Asked Questions
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