FUS Antibody

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P35637

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 499-526 from the human protein was used as the immunogen for this FUS antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the FUS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FUS antibody is available for research use only.

Storage Conditions

Aliquot the FUS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the FUS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine

Image Legend

Confocal immunofluorescent analysis of FUS antibody with MDA-MB231 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) .