IKKb Antibody
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:100-1:500
UniProt
O14920
Host
Mouse
Reactivity
Human
Immunogen
This IKKb antibody was raised using purified His-tagged recombinant full length human IKK beta.
Clonality
Monoclonal
Isotype
IgG1
Clone
62AT216
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the IKKb antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IKKb antibody is available for research use only.
Storage Conditions
Aliquot the IKKb antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the IKKb antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of IKKb antibody at 1:4000 dilution. Lane 1: HL-60 lysate; 2: HeLa lysate; 3: Jurkat lysate; Predicted molecular weight ~87 kDa.
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