CHK1 Antibody
CHK1 is a serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1,000-1:4,000
UniProt
O14757
Host
Mouse
Reactivity
Human, Mouse
Immunogen
This monoclonal CHK1 antibody was produced from mice immunized with purified recombinant protein.
Clonality
Monoclonal
Isotype
IgM
Clone
2G1D5
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CHK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CHK1 antibody is available for research use only.
Storage Conditions
Aliquot the CHK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of mouse NIH3T3 cell lysate (Lane 1) and human K562 cell lysate (2) with CHK1 antibody. Predicted molecular weight ~54kDa.
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