KLF4 Antibody

KLF4 is a transcription factor that can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

O43474

Host

Mouse

Reactivity

Human

Immunogen

Recombinant protein was used to produce this monoclonal KLF4 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

56CT5.1.6

Applications

WB, IF, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KLF4 antibody is available for research use only.

Storage Conditions

Aliquot the KLF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescence analysis with KLF4 antibody. HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.