CD33 Antibody
CD33 or Siglec-3 is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. It binds sialic acids, therefore is a member of the SIGLEC family of lectins. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:10-1:50, Western blot: 1:100-1:500
UniProt
P20138
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 337-364 from the human protein was used as the immunogen for this CD33 antibody.
Clonality
Monoclonal
Isotype
IgM
Clone
574CT16.4.4
Applications
WB, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CD33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD33 antibody is available for research use only.
Storage Conditions
Aliquot the CD33 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CD33 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Fluorescent image of A549 cell stained with CD33 antibody at 1:25. CD33 immunoreactivity is localized to the membrane.
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