KIT Antibody

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor) . Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:100, Immunofluorescence: 1:10-1:100

UniProt

P10721

Host

Mouse

Reactivity

Human

Immunogen

This KIT antibody was produced from mice immunized with human KIT recombinant protein.

Clonality

Monoclonal

Isotype

IgM

Clone

566CT8.5.4

Applications

WB, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the KIT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KIT antibody is available for research use only.

Storage Conditions

Aliquot the KIT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the KIT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent analysis of U251 cells using KIT antibody. Ab was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green) . Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red) .