Fatty Acid Synthase Antibody

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:200, Western blot: 1:100

UniProt

P49327

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 942-973 from human FASN was used as the immunogen for this Fatty Acid Synthase antibody.

Clonality

Monoclonal

Isotype

IgM

Clone

497CT15.2.5

Applications

IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Fatty Acid Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Fatty Acid Synthase antibody is available for research use only.

Storage Conditions

Aliquot the Fatty Acid Synthase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Fatty Acid Synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Fluorescent confocal image of HepG2 cells stained with Fatty Acid Synthase antibody. Alexa Fluor 488 secondary was used (green) . FASN immunosignal localizes to the cytoplasm.