UchL1 Antibody

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (Paraffin) : 1:25, Immunofluorescence: 1:25, Western blot: 1:120-1:1000

UniProt

P09936

Host

Mouse

Reactivity

Human

Immunogen

This UchL1 antibody was produced from a mouse immunized with UchL1 recombinant protein.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

346CT2.2.1

Applications

IHC, IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the UCHL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This UchL1 antibody is available for research use only.

Storage Conditions

Aliquot the UchL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the UchL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from 1) NCI-H460, 2) U266, 3) mouse Neuro-2a, rat 4) PC-12 and 5) C6 cell line and 6) mouse brain tissue using UchL1 antibody at 1:1000. Predicted molecular weight ~25 kDa.