Glucagon Antibody

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P01275

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 119-148 from the human protein was used as the immunogen for this Glucagon antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

329CT36.10.10

Applications

WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Glucagon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Glucagon antibody is available for research use only.

Storage Conditions

Aliquot the Glucagon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Glucagon antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from (1) HT29 and (2) HepG2 cell line using Glucagon antibody at 1:1000. Predicted molecular weight ~21 kDa (pro form) .