TSC2 Antibody

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:100

UniProt

P49815

Host

Mouse

Reactivity

Human, Mouse

Immunogen

This TSC2 antibody was produced from a mouse immunized with TSC2 recombinant protein.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

249CT19.1.3

Applications

WB, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TSC2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TSC2 antibody is available for research use only.

Storage Conditions

Aliquot the TSC2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the TSC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent image of HeLa cells stained with TSC2 antibody. Alexa Fluor 488 secondary (green), cytoplasmic actin (red) and nuclei counterstained with DAPI (blue) were used. TSC2 immunoreactivity is localized to the microtubules.