B-Actin Antibody

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000, Flow cytometry: 1:25

UniProt

P60709

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This b-Actin antibody was produced from a mouse immunized with ACTB recombinant protein.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

137CT26.1.1

Applications

WB, IF, IHC, FACS

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the b-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This b-Actin antibody is available for research use only.

Storage Conditions

Aliquot the b-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Chicken, Pig, Xenopus

Image Legend

Western blot analysis of b-Actin antibody in 1) K562, 2) HL-60, 3) HeLa cell line, and mouse tissues 4) spleen, 5) liver, 6) mouse NIH3T3 cell lysate, 7) mouse cerebellum and 8) mouse brain tissue lysate