Nucleolin Antibody
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50
UniProt
P19338
Host
Mouse
Reactivity
Human
Immunogen
This Nucleolin antibody was produced from mice immunized with a protein from human NCL.
Clonality
Monoclonal
Isotype
IgG1
Clone
130CT43.8.5
Applications
WB, IHC, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Nucleolin antibody is available for research use only.
Storage Conditions
Aliquot the Nucleolin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Nucleolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of Nucleolin antibody and 293 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
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