NANOG Antibody
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:100-1:250, IHC (Paraffin) : 1:10-1:50
UniProt
Q9H9S0
Host
Mouse
Reactivity
Human
Immunogen
This NANOG antibody was produced from a mouse immunized with human recombinant protein.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
60CT77.1.1
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NANOG antibody is available for research use only.
Storage Conditions
Aliquot the NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
NANOG antibody western blot analysis in K562 lysate. Predicted molecular weight: 35-45 kDa.
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