RIZ1 Antibody

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, ChIP: 1:100

UniProt

Q13029

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This RIZ1 antibody is specific and does not recognize the beta isoform (RIZ2) .

Clonality

Monoclonal

Isotype

IgG1

Clone

33AT1045

Applications

CHIP, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the RIZ1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RIZ1 antibody is available for research use only.

Storage Conditions

Aliquot the RIZ1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the RIZ1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

ChIP analysis: soluble chromatin was prepared from MCF7 cells treated/untreated with E2 for 45 min. Immunoprecipitation was performed with RIZ1 antibody. DNA extractions amplified using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.