SET07 Antibody / KMT5A
SET07 is a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1) . H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
Q9NQR1
Host
Mouse
Reactivity
Human, Mouse
Immunogen
This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.
Clonality
Monoclonal
Isotype
IgG1
Clone
43AT551.86
Applications
IHC, IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the SET07 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SET07 antibody is available for research use only.
Storage Conditions
Aliquot the SET07 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SET07 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of 293 lysate using SET07 antibody at 1:1000.
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