Beta-Tubulin Antibody (TUBB)

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:25, IHC (Paraffin) : 1:10-1:50

UniProt

P07437

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Tubulin beta chain recombinant protein was used to produce this beta-Tubulin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

87CT59.3.7

Applications

WB, IHC, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the beta-Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This beta-Tubulin antibody is available for research use only.

Storage Conditions

Aliquot the beta-Tubulin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the beta-Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Chicken, Hamster, Pig, Rat, Xenopus

Image Legend

Western blot analysis of lysate from 293, CEM, HeLa, Y79, rat PC-12 cell line, mouse brain tissue lysate using TUBB / beta-Tubulin antibody at 1:1000 for each lane.