Beta Actin Antibody
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
P60709
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
ACTB recombinant protein was used to produce this monoclonal beta Actin antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
8H10D10
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the beta-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This beta-Actin antibody is available for research use only.
Storage Conditions
Aliquot the beta-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from HepG2, HL-60 cell line (left to right) using beta-Actin antibody at 1:1000 for each lane.
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