GAPDH Antibody
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-8000, Immunofluorescence: 1:25, IHC (Paraffin) : 1:25
UniProt
P04406
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein was used to produce this monoclonal GAPDH antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
1A10A10
Applications
WB, IF, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This GAPDH antibody is available for research use only.
Storage Conditions
Aliquot the GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
GAPDH antibody WB at 1:1000 dilution. Lane 1: A431 lysate; 2: rat C6; 3: HeLa; 4: HUVEC; Predicted band size : 36 kDa.
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