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B-Tubulin Antibody

This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Tubulin beta-1 chain is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:100-1:500, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

Q9H4B7

Host

Mouse

Reactivity

Human, Mouse

Immunogen

This b-Tubulin antibody was produced from mice immunized with recombinant human TUBB1.

Clonality

Monoclonal

Isotype

IgG1

Clone

86CT4.4.4

Applications

WB, IHC, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the b-Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This b-Tubulin antibody is available for research use only.

Storage Conditions

Aliquot the b-Tubulin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the b-Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of b-Tubulin antibody and (1) mouse brain tissue, (2) Y79, (3) CEM and (4) 293 lysate.

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