HGFR Antibody (MET)
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:100
UniProt
P08581
Host
Mouse
Reactivity
Human, Mouse
Immunogen
This HGFR antibody was produced from mice immunized with purified recombinant protein encoding the catalytic domain of human MET/HGFR.
Clonality
Monoclonal
Isotype
IgG1
Clone
4AT247
Applications
IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HGFR antibody (MET) and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HGFR antibody (MET) is available for research use only.
Storage Conditions
Aliquot the HGFR antibody (MET) and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HGFR antibody (MET) may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from HepG2, human liver, HT-29 cell line (left to right) using HGFR antibody. Predicted molecular weight ~156 kDa.
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