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HGFR Antibody (MET)

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:200, Western blot: 1:100-1:500

UniProt

P08581

Host

Mouse

Reactivity

Human, Mouse

Immunogen

This HGFR antibody was produced from mice immunized with purified recombinant protein encoding the catalytic domain of human MET/HGFR.

Clonality

Monoclonal

Isotype

IgG1

Clone

4AT259

Applications

IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HGFR antibody (MET) and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HGFR antibody (MET) is available for research use only.

Storage Conditions

Aliquot the HGFR antibody (MET) and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the HGFR antibody (MET) may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent confocal image of HepG2 cells stained with HGFR antibody. HGFR is localized to the cytoplasm

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